Analysis of Mec-A Gene on Methicillin Resistant Staphylococcus aureus

Abstract

The prevalence of MRSA in various hospitals in the world ranges from 2-70% with an average of 20% .In recent decades there has been an increasing prevalence of S. aureus and MRSA in the world. Population-based studies in North America and Europe indicate the prevalence of S. aureus is between 18-30%. The overall prevalence of MRSA in Asia has reached 70%, while publications and prevalence of MRSA in Indonesia are still very limited and very difficult to obtain. The prevalence of MRSA in Atmajaya Hospital, Jakarta, reached 47%. Yuwono also reported in 2010 MRSA at Dr. RSUP. Moh. Hoesin Palembang reached 46%. Amplification were carried out under PCR conditions as a result of optimization, namely the results of PCR optimization were carried out for 30 cycles with the following thermal conditions. Denaturation phase at 94^oC for 30 seconds, annealing at 52.0^oC for 30 seconds, and extension at 72^oC for 1 minute. Amplification results showed that of the 30 samples only 90% (27 samples) were detected to have the Mec-A gene, 10% (3 samples) were negative.

Keywords: Mec-A; gene; Methicillin Resistant Staphylococcus aureus